Leica DMi8 TIRF Widefield Fluorescence Microscope & Andor SRRF Camera
Only when using the SRRF camera
Microscopic detection of luminescent signals was enabled by an ANDOR IXON ULTRA EMCCD SRRF camera, funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 261193037
| Magnification | 10 | 40 | 63 | 100 TiRF |
| NA | 0,32 | 0,60 | 1,40 | 1,47 |
| Immersion | dry | dry | oil | oil |
| λ exc (nm) | 488 TiRF laser | 561 TiRF laser | sola light engine | 405 / 60 | 480 / 40 | 546 / 10 | 620 / 60 | |
| λ em (nm) or Filter | TriWF-TiRF: ex 400/22+488/10+561/10; dm 420+505+572; em 448/48; 525/32; 611/70 | GFP-TiRF: ex 475/40; dm 500; em 530/50 | Y3-TiRF: ex555/25; dm575; em605/55 (add-on) | CFP/YFP-TiRF:ex405/10+488/10; dm 418+500; em 545/50+ LP500 | LED-405: ex405/60; dm 455; em 470/40 | FiTC: ex480/40; dm505; em527/30 | RHOD: ex546/10; dm560; em585/40 (add-on) | Y5: ex620/60; dm660; em700/75 |
Fluorescence Filter:
- 405 – 470/40
- FITC – 527/30
- Rhod – 585/40
- Y5 – 700/75
Additional features:
- Infinity port
- sCMOS camera
- Hamamatsu Orca flash 4.0
- SRRF:
Andor Ixon Ultra EMCCD
BiophotonicsPC